Analysis Note

Western blotting by primary antibody rat anti L1 (Millipore Corp, cat.# MAB5272) and secondary antibody goat anti rat, HRP (Millipore Corp, cat.# AP183P),Single band expected at ~ 200 kDa

Application

Used at 5 µg/ml (see references 1 and 2).
Optimal working concentration must be determined by end user.

General description

L1, a cell adhesion molecule of the Ig superfamily (IgCAM) plays a critical role in the formation of neuronal networks. This is reflected by the variety of clinical signs associated with the X-linked recessive neurological disorder that is caused by mutations in the L1 gene. L1 regulates the formation of axon fascicles and promotes neurite outgrowth through interaction with a wide spectrum of binding partners including cell adhesion molecules and extra-cellular matrix components. L1 participates in the signaling of a secreted guidance cue of the Semaphorin family, Sema3A. Three types of experimental evidence support L1 as a key component of the Sema3A receptor complex.

Chimeric protein of Human neurite growth protein L1 and Human IgG-Fc γ domain purified using protein A-agarose (Millipore Corp, cat. # 16-125). MW ~ 200 kDa.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

100 µg of recombinant protein in 100µl of 1X PBS, 20%(v/v) glycerol. Frozen solution.